Cross-reactive antibodies in convalescent SARS patients' sera against the emerging novel human coronavirus EMC (2012) by both immunofluorescent and neutralizing antibody tests
Identifieur interne : 001C09 ( Main/Exploration ); précédent : 001C08; suivant : 001C10Cross-reactive antibodies in convalescent SARS patients' sera against the emerging novel human coronavirus EMC (2012) by both immunofluorescent and neutralizing antibody tests
Auteurs : Kwok-Hung Chan [Hong Kong] ; Jasper Fuk-Woo Chan [Hong Kong] ; Herman Tse [Hong Kong] ; HONGLIN CHEN [Hong Kong] ; Candy Choi-Yi Lau [Hong Kong] ; Jian-Piao Cai [Hong Kong] ; Alan Ka-Lun Tsang [Hong Kong] ; XINCAI XIAO [République populaire de Chine] ; Kelvin Kai-Wang To [Hong Kong] ; Susanna Kar-Pui Lau [Hong Kong] ; Patrick Chiu-Yat Woo [Hong Kong] ; Bo-Jiang Zheng [Hong Kong] ; MING WANG [République populaire de Chine] ; Kwok-Yung Yuen [Hong Kong]Source :
- The Journal of infection [ 0163-4453 ] ; 2013.
Descripteurs français
- KwdFr :
- Adulte, Adulte d'âge moyen, Animaux, Anticorps antiviraux (sang), Anticorps neutralisants (sang), Chine, Coronaviridae (immunologie), Femelle, Humains, Jeune adulte, Mâle, Réactions croisées, Sujet âgé, Syndrome respiratoire aigu sévère (immunologie), Technique d'immunofluorescence indirecte (), Tests de neutralisation (), Études séroépidémiologiques.
- MESH :
- immunologie : Coronaviridae, Syndrome respiratoire aigu sévère.
- sang : Anticorps antiviraux, Anticorps neutralisants.
- Pascal (Inist)
- Adulte, Adulte d'âge moyen, Animaux, Chine, Femelle, Humains, Jeune adulte, Mâle, Réactions croisées, Sujet âgé, Syndrome respiratoire aigu sévère, Réaction croisée, Anticorps, Sérum, Homme, Coronavirus, Neutralisation, Technique d'immunofluorescence indirecte, Tests de neutralisation, Études séroépidémiologiques.
- Wicri :
- geographic : République populaire de Chine.
- topic : Homme.
English descriptors
- KwdEn :
- Adult, Aged, Animals, Antibodies, Neutralizing (blood), Antibodies, Viral (blood), Antibody, China, Coronaviridae (immunology), Coronavirus, Cross Reactions, Cross reaction, Female, Fluorescent Antibody Technique, Indirect (methods), Human, Humans, Male, Middle Aged, Neutralization, Neutralization Tests (methods), Seroepidemiologic Studies, Serum, Severe Acute Respiratory Syndrome (immunology), Severe acute respiratory syndrome, Young Adult.
- MESH :
- chemical , blood : Antibodies, Neutralizing, Antibodies, Viral.
- geographic : China.
- immunology : Coronaviridae, Severe Acute Respiratory Syndrome.
- methods : Fluorescent Antibody Technique, Indirect, Neutralization Tests.
- Adult, Aged, Animals, Cross Reactions, Female, Humans, Male, Middle Aged, Seroepidemiologic Studies, Young Adult.
Abstract
Objectives: A severe acute respiratory syndrome (SARS)-like disease due to a novel betacoronavirus, human coronavirus EMC (HCoV-EMC), has emerged recently. HCoV-EMC is phylogenetically closely related to Tylonycteris-bat-coronavirus-HKU4 and Pipistrellus-bat-coronavirus-HKU5 in Hong Kong. We conducted a seroprevalence study on archived sera from 94 game-food animal handlers at a wild life market, 28 SARS patients, and 152 healthy blood donors in Southern China to assess the zoonotic potential and evidence for intrusion of HCoV-EMC and related viruses into humans. Methods: Anti-HCoV-EMC and anti-SARS-CoV antibodies were detected using screening indirect immunofluorescence (IF) and confirmatory neutralizing antibody tests. Results: Two (2.1%) animal handlers had IF antibody titer of ≥1:20 against both HCoV-EMC and SARS-CoV with neutralizing antibody titer of <1:10. No blood donor had antibody against either virus. Surprisingly, 17/28 (60.7%) of SARS patients had significant IF antibody titers with 7/28 (25%) having anti-HCoV-EMC neutralizing antibodies at low titers which significantly correlated with that of HCoV-OC43. Bioinformatics analysis demonstrated a significant B-cell epitope overlapping the heptad repeat-2 region of Spike protein. Virulence of SARS-CoV over other betacoronaviruses may boost cross-reactive neutralizing antibodies against other betacoronaviruses. Conclusions: Convalescent SARS sera may contain cross-reactive antibodies against other betacoronaviruses and confound seroprevalence study for HCoV-EMC.
Url:
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 000024
- to stream PascalFrancis, to step Curation: 000963
- to stream PascalFrancis, to step Checkpoint: 000023
- to stream Main, to step Merge: 001C19
- to stream Pmc, to step Corpus: 001181
- to stream Pmc, to step Curation: 001181
- to stream Pmc, to step Checkpoint: 000999
- to stream PubMed, to step Corpus: 001218
- to stream PubMed, to step Curation: 001218
- to stream PubMed, to step Checkpoint: 001205
- to stream Ncbi, to step Merge: 002654
- to stream Ncbi, to step Curation: 002654
- to stream Ncbi, to step Checkpoint: 002654
- to stream Main, to step Merge: 001978
- to stream Main, to step Curation: 001C09
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Cross-reactive antibodies in convalescent SARS patients' sera against the emerging novel human coronavirus EMC (2012) by both immunofluorescent and neutralizing antibody tests</title><author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</wicri:noRegion></affiliation></author><author><name sortKey="Chan, Jasper Fuk Woo" sort="Chan, Jasper Fuk Woo" uniqKey="Chan J" first="Jasper Fuk-Woo" last="Chan">Jasper Fuk-Woo Chan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</wicri:noRegion></affiliation></author><author><name sortKey="Tse, Herman" sort="Tse, Herman" uniqKey="Tse H" first="Herman" last="Tse">Herman Tse</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Research Centre of Infection and Immunology, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Research Centre of Infection and Immunology, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Carol Yu Centre for Infection, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Carol Yu Centre for Infection, The University of Hong Kong</wicri:noRegion></affiliation></author><author><name sortKey="Honglin Chen" sort="Honglin Chen" uniqKey="Honglin Chen" last="Honglin Chen">HONGLIN CHEN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Research Centre of Infection and Immunology, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Research Centre of Infection and Immunology, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Carol Yu Centre for Infection, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Carol Yu Centre for Infection, The University of Hong Kong</wicri:noRegion></affiliation></author><author><name sortKey="Lau, Candy Choi Yi" sort="Lau, Candy Choi Yi" uniqKey="Lau C" first="Candy Choi-Yi" last="Lau">Candy Choi-Yi Lau</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</wicri:noRegion></affiliation></author><author><name sortKey="Cai, Jian Piao" sort="Cai, Jian Piao" uniqKey="Cai J" first="Jian-Piao" last="Cai">Jian-Piao Cai</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</wicri:noRegion></affiliation></author><author><name sortKey="Tsang, Alan Ka Lun" sort="Tsang, Alan Ka Lun" uniqKey="Tsang A" first="Alan Ka-Lun" last="Tsang">Alan Ka-Lun Tsang</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</wicri:noRegion></affiliation></author><author><name sortKey="Xincai Xiao" sort="Xincai Xiao" uniqKey="Xincai Xiao" last="Xincai Xiao">XINCAI XIAO</name><affiliation wicri:level="3"><inist:fA14 i1="05"><s1>Guangzhou Center for Disease Control and Prevention</s1><s2>Guangzhou</s2><s3>CHN</s3><sZ>8 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="To, Kelvin Kai Wang" sort="To, Kelvin Kai Wang" uniqKey="To K" first="Kelvin Kai-Wang" last="To">Kelvin Kai-Wang To</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Research Centre of Infection and Immunology, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Research Centre of Infection and Immunology, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Carol Yu Centre for Infection, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Carol Yu Centre for Infection, The University of Hong Kong</wicri:noRegion></affiliation></author><author><name sortKey="Lau, Susanna Kar Pui" sort="Lau, Susanna Kar Pui" uniqKey="Lau S" first="Susanna Kar-Pui" last="Lau">Susanna Kar-Pui Lau</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Research Centre of Infection and Immunology, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Research Centre of Infection and Immunology, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Carol Yu Centre for Infection, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Carol Yu Centre for Infection, The University of Hong Kong</wicri:noRegion></affiliation></author><author><name sortKey="Woo, Patrick Chiu Yat" sort="Woo, Patrick Chiu Yat" uniqKey="Woo P" first="Patrick Chiu-Yat" last="Woo">Patrick Chiu-Yat Woo</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Research Centre of Infection and Immunology, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Research Centre of Infection and Immunology, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Carol Yu Centre for Infection, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Carol Yu Centre for Infection, The University of Hong Kong</wicri:noRegion></affiliation></author><author><name sortKey="Zheng, Bo Jiang" sort="Zheng, Bo Jiang" uniqKey="Zheng B" first="Bo-Jiang" last="Zheng">Bo-Jiang Zheng</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Research Centre of Infection and Immunology, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Research Centre of Infection and Immunology, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Carol Yu Centre for Infection, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Carol Yu Centre for Infection, The University of Hong Kong</wicri:noRegion></affiliation></author><author><name sortKey="Ming Wang" sort="Ming Wang" uniqKey="Ming Wang" last="Ming Wang">MING WANG</name><affiliation wicri:level="3"><inist:fA14 i1="05"><s1>Guangzhou Center for Disease Control and Prevention</s1><s2>Guangzhou</s2><s3>CHN</s3><sZ>8 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Research Centre of Infection and Immunology, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Research Centre of Infection and Immunology, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Carol Yu Centre for Infection, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Carol Yu Centre for Infection, The University of Hong Kong</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">13-0231386</idno><date when="2013">2013</date><idno type="stanalyst">PASCAL 13-0231386 INIST</idno><idno type="RBID">Pascal:13-0231386</idno><idno type="wicri:Area/PascalFrancis/Corpus">000024</idno><idno type="wicri:Area/PascalFrancis/Curation">000963</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000023</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000023</idno><idno type="wicri:doubleKey">0163-4453:2013:Chan K:cross:reactive:antibodies</idno><idno type="wicri:Area/Main/Merge">001C19</idno><idno type="wicri:source">PMC</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112694</idno><idno type="RBID">PMC:7112694</idno><idno type="wicri:Area/Pmc/Corpus">001181</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001181</idno><idno type="wicri:Area/Pmc/Curation">001181</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001181</idno><idno type="wicri:Area/Pmc/Checkpoint">000999</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000999</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:23583636</idno><idno type="wicri:Area/PubMed/Corpus">001218</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001218</idno><idno type="wicri:Area/PubMed/Curation">001218</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001218</idno><idno type="wicri:Area/PubMed/Checkpoint">001205</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001205</idno><idno type="wicri:Area/Ncbi/Merge">002654</idno><idno type="wicri:Area/Ncbi/Curation">002654</idno><idno type="wicri:Area/Ncbi/Checkpoint">002654</idno><idno type="wicri:doubleKey">0163-4453:2013:Chan K:cross:reactive:antibodies</idno><idno type="wicri:Area/Main/Merge">001978</idno><idno type="wicri:Area/Main/Curation">001C09</idno><idno type="wicri:Area/Main/Exploration">001C09</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Cross-reactive antibodies in convalescent SARS patients' sera against the emerging novel human coronavirus EMC (2012) by both immunofluorescent and neutralizing antibody tests</title><author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</wicri:noRegion></affiliation></author><author><name sortKey="Chan, Jasper Fuk Woo" sort="Chan, Jasper Fuk Woo" uniqKey="Chan J" first="Jasper Fuk-Woo" last="Chan">Jasper Fuk-Woo Chan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</wicri:noRegion></affiliation></author><author><name sortKey="Tse, Herman" sort="Tse, Herman" uniqKey="Tse H" first="Herman" last="Tse">Herman Tse</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Research Centre of Infection and Immunology, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Research Centre of Infection and Immunology, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Carol Yu Centre for Infection, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Carol Yu Centre for Infection, The University of Hong Kong</wicri:noRegion></affiliation></author><author><name sortKey="Honglin Chen" sort="Honglin Chen" uniqKey="Honglin Chen" last="Honglin Chen">HONGLIN CHEN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Research Centre of Infection and Immunology, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Research Centre of Infection and Immunology, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Carol Yu Centre for Infection, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Carol Yu Centre for Infection, The University of Hong Kong</wicri:noRegion></affiliation></author><author><name sortKey="Lau, Candy Choi Yi" sort="Lau, Candy Choi Yi" uniqKey="Lau C" first="Candy Choi-Yi" last="Lau">Candy Choi-Yi Lau</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</wicri:noRegion></affiliation></author><author><name sortKey="Cai, Jian Piao" sort="Cai, Jian Piao" uniqKey="Cai J" first="Jian-Piao" last="Cai">Jian-Piao Cai</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</wicri:noRegion></affiliation></author><author><name sortKey="Tsang, Alan Ka Lun" sort="Tsang, Alan Ka Lun" uniqKey="Tsang A" first="Alan Ka-Lun" last="Tsang">Alan Ka-Lun Tsang</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</wicri:noRegion></affiliation></author><author><name sortKey="Xincai Xiao" sort="Xincai Xiao" uniqKey="Xincai Xiao" last="Xincai Xiao">XINCAI XIAO</name><affiliation wicri:level="3"><inist:fA14 i1="05"><s1>Guangzhou Center for Disease Control and Prevention</s1><s2>Guangzhou</s2><s3>CHN</s3><sZ>8 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="To, Kelvin Kai Wang" sort="To, Kelvin Kai Wang" uniqKey="To K" first="Kelvin Kai-Wang" last="To">Kelvin Kai-Wang To</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Research Centre of Infection and Immunology, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Research Centre of Infection and Immunology, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Carol Yu Centre for Infection, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Carol Yu Centre for Infection, The University of Hong Kong</wicri:noRegion></affiliation></author><author><name sortKey="Lau, Susanna Kar Pui" sort="Lau, Susanna Kar Pui" uniqKey="Lau S" first="Susanna Kar-Pui" last="Lau">Susanna Kar-Pui Lau</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Research Centre of Infection and Immunology, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Research Centre of Infection and Immunology, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Carol Yu Centre for Infection, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Carol Yu Centre for Infection, The University of Hong Kong</wicri:noRegion></affiliation></author><author><name sortKey="Woo, Patrick Chiu Yat" sort="Woo, Patrick Chiu Yat" uniqKey="Woo P" first="Patrick Chiu-Yat" last="Woo">Patrick Chiu-Yat Woo</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Research Centre of Infection and Immunology, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Research Centre of Infection and Immunology, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Carol Yu Centre for Infection, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Carol Yu Centre for Infection, The University of Hong Kong</wicri:noRegion></affiliation></author><author><name sortKey="Zheng, Bo Jiang" sort="Zheng, Bo Jiang" uniqKey="Zheng B" first="Bo-Jiang" last="Zheng">Bo-Jiang Zheng</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Research Centre of Infection and Immunology, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Research Centre of Infection and Immunology, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Carol Yu Centre for Infection, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Carol Yu Centre for Infection, The University of Hong Kong</wicri:noRegion></affiliation></author><author><name sortKey="Ming Wang" sort="Ming Wang" uniqKey="Ming Wang" last="Ming Wang">MING WANG</name><affiliation wicri:level="3"><inist:fA14 i1="05"><s1>Guangzhou Center for Disease Control and Prevention</s1><s2>Guangzhou</s2><s3>CHN</s3><sZ>8 aut.</sZ><sZ>13 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Research Centre of Infection and Immunology, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Research Centre of Infection and Immunology, The University of Hong Kong</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Carol Yu Centre for Infection, The University of Hong Kong</s1><s3>HKG</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Carol Yu Centre for Infection, The University of Hong Kong</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">The Journal of infection</title><title level="j" type="abbreviated">J. infect.</title><idno type="ISSN">0163-4453</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">The Journal of infection</title><title level="j" type="abbreviated">J. infect.</title><idno type="ISSN">0163-4453</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Aged</term><term>Animals</term><term>Antibodies, Neutralizing (blood)</term><term>Antibodies, Viral (blood)</term><term>Antibody</term><term>China</term><term>Coronaviridae (immunology)</term><term>Coronavirus</term><term>Cross Reactions</term><term>Cross reaction</term><term>Female</term><term>Fluorescent Antibody Technique, Indirect (methods)</term><term>Human</term><term>Humans</term><term>Male</term><term>Middle Aged</term><term>Neutralization</term><term>Neutralization Tests (methods)</term><term>Seroepidemiologic Studies</term><term>Serum</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe acute respiratory syndrome</term><term>Young Adult</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte</term><term>Adulte d'âge moyen</term><term>Animaux</term><term>Anticorps antiviraux (sang)</term><term>Anticorps neutralisants (sang)</term><term>Chine</term><term>Coronaviridae (immunologie)</term><term>Femelle</term><term>Humains</term><term>Jeune adulte</term><term>Mâle</term><term>Réactions croisées</term><term>Sujet âgé</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Technique d'immunofluorescence indirecte ()</term><term>Tests de neutralisation ()</term><term>Études séroépidémiologiques</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Neutralizing</term><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>China</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Coronaviridae</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Coronaviridae</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Fluorescent Antibody Technique, Indirect</term><term>Neutralization Tests</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term><term>Anticorps neutralisants</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Aged</term><term>Animals</term><term>Cross Reactions</term><term>Female</term><term>Humans</term><term>Male</term><term>Middle Aged</term><term>Seroepidemiologic Studies</term><term>Young Adult</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Adulte</term><term>Adulte d'âge moyen</term><term>Animaux</term><term>Chine</term><term>Femelle</term><term>Humains</term><term>Jeune adulte</term><term>Mâle</term><term>Réactions croisées</term><term>Sujet âgé</term><term>Syndrome respiratoire aigu sévère</term><term>Réaction croisée</term><term>Anticorps</term><term>Sérum</term><term>Homme</term><term>Coronavirus</term><term>Neutralisation</term><term>Technique d'immunofluorescence indirecte</term><term>Tests de neutralisation</term><term>Études séroépidémiologiques</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>République populaire de Chine</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Objectives: A severe acute respiratory syndrome (SARS)-like disease due to a novel betacoronavirus, human coronavirus EMC (HCoV-EMC), has emerged recently. HCoV-EMC is phylogenetically closely related to Tylonycteris-bat-coronavirus-HKU4 and Pipistrellus-bat-coronavirus-HKU5 in Hong Kong. We conducted a seroprevalence study on archived sera from 94 game-food animal handlers at a wild life market, 28 SARS patients, and 152 healthy blood donors in Southern China to assess the zoonotic potential and evidence for intrusion of HCoV-EMC and related viruses into humans. Methods: Anti-HCoV-EMC and anti-SARS-CoV antibodies were detected using screening indirect immunofluorescence (IF) and confirmatory neutralizing antibody tests. Results: Two (2.1%) animal handlers had IF antibody titer of ≥1:20 against both HCoV-EMC and SARS-CoV with neutralizing antibody titer of <1:10. No blood donor had antibody against either virus. Surprisingly, 17/28 (60.7%) of SARS patients had significant IF antibody titers with 7/28 (25%) having anti-HCoV-EMC neutralizing antibodies at low titers which significantly correlated with that of HCoV-OC43. Bioinformatics analysis demonstrated a significant B-cell epitope overlapping the heptad repeat-2 region of Spike protein. Virulence of SARS-CoV over other betacoronaviruses may boost cross-reactive neutralizing antibodies against other betacoronaviruses. Conclusions: Convalescent SARS sera may contain cross-reactive antibodies against other betacoronaviruses and confound seroprevalence study for HCoV-EMC.</div></front></TEI><affiliations><list><country><li>Hong Kong</li><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Jiangmen</li></settlement></list><tree><country name="Hong Kong"><noRegion><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name></noRegion><name sortKey="Cai, Jian Piao" sort="Cai, Jian Piao" uniqKey="Cai J" first="Jian-Piao" last="Cai">Jian-Piao Cai</name><name sortKey="Chan, Jasper Fuk Woo" sort="Chan, Jasper Fuk Woo" uniqKey="Chan J" first="Jasper Fuk-Woo" last="Chan">Jasper Fuk-Woo Chan</name><name sortKey="Honglin Chen" sort="Honglin Chen" uniqKey="Honglin Chen" last="Honglin Chen">HONGLIN CHEN</name><name sortKey="Honglin Chen" sort="Honglin Chen" uniqKey="Honglin Chen" last="Honglin Chen">HONGLIN CHEN</name><name sortKey="Honglin Chen" sort="Honglin Chen" uniqKey="Honglin Chen" last="Honglin Chen">HONGLIN CHEN</name><name sortKey="Honglin Chen" sort="Honglin Chen" uniqKey="Honglin Chen" last="Honglin Chen">HONGLIN CHEN</name><name sortKey="Lau, Candy Choi Yi" sort="Lau, Candy Choi Yi" uniqKey="Lau C" first="Candy Choi-Yi" last="Lau">Candy Choi-Yi Lau</name><name sortKey="Lau, Susanna Kar Pui" sort="Lau, Susanna Kar Pui" uniqKey="Lau S" first="Susanna Kar-Pui" last="Lau">Susanna Kar-Pui Lau</name><name sortKey="Lau, Susanna Kar Pui" sort="Lau, Susanna Kar Pui" uniqKey="Lau S" first="Susanna Kar-Pui" last="Lau">Susanna Kar-Pui Lau</name><name sortKey="Lau, Susanna Kar Pui" sort="Lau, Susanna Kar Pui" uniqKey="Lau S" first="Susanna Kar-Pui" last="Lau">Susanna Kar-Pui Lau</name><name sortKey="Lau, Susanna Kar Pui" sort="Lau, Susanna Kar Pui" uniqKey="Lau S" first="Susanna Kar-Pui" last="Lau">Susanna Kar-Pui Lau</name><name sortKey="To, Kelvin Kai Wang" sort="To, Kelvin Kai Wang" uniqKey="To K" first="Kelvin Kai-Wang" last="To">Kelvin Kai-Wang To</name><name sortKey="To, Kelvin Kai Wang" sort="To, Kelvin Kai Wang" uniqKey="To K" first="Kelvin Kai-Wang" last="To">Kelvin Kai-Wang To</name><name sortKey="To, Kelvin Kai Wang" sort="To, Kelvin Kai Wang" uniqKey="To K" first="Kelvin Kai-Wang" last="To">Kelvin Kai-Wang To</name><name sortKey="To, Kelvin Kai Wang" sort="To, Kelvin Kai Wang" uniqKey="To K" first="Kelvin Kai-Wang" last="To">Kelvin Kai-Wang To</name><name sortKey="Tsang, Alan Ka Lun" sort="Tsang, Alan Ka Lun" uniqKey="Tsang A" first="Alan Ka-Lun" last="Tsang">Alan Ka-Lun Tsang</name><name sortKey="Tse, Herman" sort="Tse, Herman" uniqKey="Tse H" first="Herman" last="Tse">Herman Tse</name><name sortKey="Tse, Herman" sort="Tse, Herman" uniqKey="Tse H" first="Herman" last="Tse">Herman Tse</name><name sortKey="Tse, Herman" sort="Tse, Herman" uniqKey="Tse H" first="Herman" last="Tse">Herman Tse</name><name sortKey="Tse, Herman" sort="Tse, Herman" uniqKey="Tse H" first="Herman" last="Tse">Herman Tse</name><name sortKey="Woo, Patrick Chiu Yat" sort="Woo, Patrick Chiu Yat" uniqKey="Woo P" first="Patrick Chiu-Yat" last="Woo">Patrick Chiu-Yat Woo</name><name sortKey="Woo, Patrick Chiu Yat" sort="Woo, Patrick Chiu Yat" uniqKey="Woo P" first="Patrick Chiu-Yat" last="Woo">Patrick Chiu-Yat Woo</name><name sortKey="Woo, Patrick Chiu Yat" sort="Woo, Patrick Chiu Yat" uniqKey="Woo P" first="Patrick Chiu-Yat" last="Woo">Patrick Chiu-Yat Woo</name><name sortKey="Woo, Patrick Chiu Yat" sort="Woo, Patrick Chiu Yat" uniqKey="Woo P" first="Patrick Chiu-Yat" last="Woo">Patrick Chiu-Yat Woo</name><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><name sortKey="Zheng, Bo Jiang" sort="Zheng, Bo Jiang" uniqKey="Zheng B" first="Bo-Jiang" last="Zheng">Bo-Jiang Zheng</name><name sortKey="Zheng, Bo Jiang" sort="Zheng, Bo Jiang" uniqKey="Zheng B" first="Bo-Jiang" last="Zheng">Bo-Jiang Zheng</name><name sortKey="Zheng, Bo Jiang" sort="Zheng, Bo Jiang" uniqKey="Zheng B" first="Bo-Jiang" last="Zheng">Bo-Jiang Zheng</name><name sortKey="Zheng, Bo Jiang" sort="Zheng, Bo Jiang" uniqKey="Zheng B" first="Bo-Jiang" last="Zheng">Bo-Jiang Zheng</name></country><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Xincai Xiao" sort="Xincai Xiao" uniqKey="Xincai Xiao" last="Xincai Xiao">XINCAI XIAO</name></region><name sortKey="Ming Wang" sort="Ming Wang" uniqKey="Ming Wang" last="Ming Wang">MING WANG</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001C09 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001C09 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= Pascal:13-0231386
|texte= Cross-reactive antibodies in convalescent SARS patients' sera against the emerging novel human coronavirus EMC (2012) by both immunofluorescent and neutralizing antibody tests
}}
| This area was generated with Dilib version V0.6.33. |  |